14 May A microfluidic platform to investigate effect of mechanobiological cues on the dynamics of angiogenesis
Mechanobiological cues can be of crucial importance after an injury for regulating wound angiogenesis. However, mechanoregulation of angiogenesis is not fully understood in wound healing. Microfluidics has shown to be the optimal choice when it comes to mimicking the cellular microenvironment and investigating the effect of mechanical, chemical, electrical, etc. on cellular activities. Here, in a recent publication in Nature Communications, a research group developed a microfluidic chip for investigating the effect of intraluminal pressure on wound angiogenesis. By mimicking the blood flow-driven intraluminal pressure (IP) in a microfluidic environment, the team could analyze the dynamics of angiogenesis upstream and downstream of the wound.
“Angiogenesis is regulated in coordinated fashion by chemical and mechanical cues acting on endothelial cells (ECs). However, the mechanobiological mechanisms of angiogenesis remain unknown. Herein, we demonstrate a crucial role of blood flow-driven intraluminal pressure (IP) in regulating wound angiogenesis. During wound angiogenesis, blood flow-driven IP loading inhibits elongation of injured blood vessels located at sites upstream from blood flow, while downstream injured vessels actively elongate.“, the authors explained.
“In this study, we uncovered a novel molecular mechanism governing the mechanical regulation of EC behavior during wound angiogenesis. By exploiting a recently developed live imaging system for adult zebrafish20, we analyzed the dynamics of angiogenic EC behavior during wound healing and surprisingly discovered that elongation of severed blood vessels is preferentially induced downstream from blood flow, whereas blood flow-driven intraluminal pressure (IP) loading suppresses elongation of upstream injured vessels by inducing EC stretching.“, the authors explained.
Figures were reproduced Yuge, S., Nishiyama, K., Arima, Y. et al. Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Nat Commun 13, 2594 (2022). https://doi.org/10.1038/s41467-022-30197-8 under Creative Commons Attribution 4.0 International License.