28 May Monolayer assembly of barcoded microparticles for multiplexed immunoassays
One of the reasons that some of the microfluidic products that show promise in research settings never find their way to the market lies in the complicated operation procedure. The simpler the microfluidic device the higher the chance of that product being well-received by the market. For this week’s research highlight, we have selected one such advancement. In this communication article published in the Lab On a Chip journal, a research team reported a microfluidic chip that simplifies the assembly of barcoded planar microparticles for multiplexed immunoassay. In the proposed method, the team showed that by using only a pipette, they could assemble a monolayer of fluorescent barcoded particles in a microfluidic chip.
“Barcoded planar microparticles are suitable for developing cost-efficient multiplexed assays, but the robustness and efficiency of the readout process still needs improvement. Here, we designed a one-step microparticle assembling chip that produces efficient and accurate multiplex immunoassay readout results. Our design was also compatible with injection molding for mass production.“, the authors explained.
The proposed microfluidic chip is made with PDMS using conventional microfluidic microfabrication techniques. The microfluidic chip was designed such that it could be operated without the need for pumps and valves. The barcoded microparticles could be guided through the device by a pipetting-induced capillary flow. The stopping pillar downstream of the microfluidic chip ensured that the microparticles did not escape the chip. The microfluidic chip was first optimized to find the channel height that results in a monolayer assembly. Upon optimization, 8 parallel microfluidic channels were placed alongside each other to increase the readout throughput. Fluorescent imaging of the microfluidic chip indicated the successful formation of a monolayer of fluorescent microparticles.
“Other than the chip and a pipette, our strategy doesn’t require cumbersome syringe pumps or other fluidic controlling modules. We expect that this chip design will find utility in point-of-care applications where tools are encouraged to be simple yet have high throughput, and multiplexing capacity is required.“, the authors concluded.
Figures are reproduced were reproduced from S. Bae, D. Lee, H. Na, J. Jang and S. Kwon, Lab Chip, 2022, Advance Article , DOI: 10.1039/D2LC00174H with permission from the Royal Society of Chemistry.
Read the original article: One-step assembly of barcoded planar microparticles for efficient readout of multiplexed immunoassay