24 Jan Droplet microfluidic-assisted bioprinting of functional living materials
“Living materials bring together material science and biology to allow the engineering and augmenting of living systems with novel functionalities. Bioprinting promises accurate control over the formation of such complex materials through programmable deposition of cells in soft materials, but current approaches had limited success in fine-tuning cell microenvironments while generating robust macroscopic morphologies. Here, we address this challenge through the use of core-shell microgel ink to decouple cell microenvironments from the structural shell for further processing. Cells are microfluidically immobilized in the viscous core that can promote the formation of both microbial populations and mammalian cellular spheroids, followed by interparticle annealing to give covalently stabilized functional scaffolds with controlled microporosity. The results show that the core-shell strategy mitigates cell leakage while affording a favorable environment for cell culture. Furthermore, we demonstrate that different microbial consortia can be printed into scaffolds for a range of applications. By compartmentalizing microbial consortia in separate microgels, the collective bioprocessing capability of the scaffold is significantly enhanced, shedding light on strategies to augment living materials with bioprocessing capabilities.”
Figures and the abstract are reproduced from Ou, Y., Cao, S., Zhang, Y. et al. Bioprinting microporous functional living materials from protein-based core-shell microgels. Nat Commun 14, 322 (2023). https://doi.org/10.1038/s41467-022-35140-5 under a Creative Commons Attribution 4.0 International License.
Read the original article: Bioprinting microporous functional living materials from protein-based core-shell microgels